Formalin 10% neutral buffered (ready to use)

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Primary container: container in PP silkscreened. Serigraphy resistant to water, alcohol and solvents. Pressure cap with a seal. Secondary container: plateau with shrink film + label. Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Specifications pH 7,0 -7,2 ± 0,2 Density 1.003 Buffer molarity 0.05 M Application Universal fixative for histological specimens. Principle The 10% formalin neutral buffered (equivalent to an aqueous solution of 4% formaldehyde) is the fixative most commonly used in the histopathological routine. The interaction between formaldehyde and functional groups present in tissue macromolecules (proteins and nucleic acids) occurs according to the following scheme:  formation of methylene glycol: the molecule of formaldehyde in water gives rise to the following equilibrium: CH2O + H2O = CH2(OH)2  The methylene glycol is the chemical species that interacts primarily with the functional groups present in the side chains of the proteins and with acids stabilizing the nuclear structure.  secondarily formaldehyde form crosslinks between the free amino groups present in the side chains of amino acids. Fixation technique 1) Volume ratio specimen/ fixative 1 : 50 2) Specimen thickness 1 cm max 3) Fixation time at room temperature: for specimens up to 5 mm 5 hours, for greater thickness 1-2 days.