Gordon-Sweet for reticulum fibres 100 test - Special Stains

Download Product's Datasheet

This method produces a selective evident impregnation in a very short time thanks to two factors: the preliminary impregnation with an iron salt and the use as silver source of an unstable diaminic complex (ammoniacal solution), which is more reactive than silver nitrate.

Method:

1) Bring the section to distilled water.
2) Put on the section 5 drops of reagent A and 5 drops of reagent B: leave to act 5 minutes.
3) Wash the slide in distilled water.
4) Put on the section 10 drops of reagent C: leave to act 1 minute.
5) Double washing in distilled water.
6) Put on the section 10 drops of reagent D: leave to act 3 minutes.
7) Double washing in distilled water.
8) Put on the section 10 drops of reagent E: leave to act 3 minutes.
9) Wash in distilled water.
10)Put on the section 10 drops of reagent F: leave to act 5 minutes.
11)Double washing in distilled water.
12)Put on the section 10 drops of reagent G: leave to act 2 minutes.
13)Wash in distilled water.
14)Put on the section 10 drops of reagent H: leave to act 2 minutes.
15)Wash in distilled water.
16)Put on the section 10 drops of reagent I: leave to act 5 minutes.
17)Dehydrate through ascending alcohols: clear in xylene and mount.