Grocott 120 test - Special Stains

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Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate fungi in tissue sections.

Method:

1) Bring section to distilled water.
2) Put on the section 10 drops of reagent A: leave to act 20 minutes.
3) Wash in running tap water for a few seconds.
4) Put on the section 10 drops of reagent B for 1 minute.
5) Wash in tap water for 5 minutes.
6) Rinse in 4 changes of distilled water.
7) In one of the enclosed slide-holder put 17 ml of distilled water and add 20 drops of reagent C, 10 drops of reagent D and 20 drops of reagent E. Stir briefly with a glass stick previously washed in distilled water.
8) Put the section in the holder and incubate in oven at 60 °C for 1 hour.
9) Take out the holder from oven and let it cool for 10 minutes.
10)Wash in 6 changes of distilled water.
11)Put on the section 10 drops of reagent F: leave to act for 3 minutes.
12)Wash in distilled water.
13)Put on the section 10 drops of reagent G: leave to act 5 minutes.
14)Wash in tap water.
15)Put on the section 10 drops of reagent H for 30 seconds.
16)Dehydrate through ascending alcohols, clear in xylene and moun.