May Grunwald Pappenheim Staining Solution

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Aim: Product for the preparation of cyto-histological samples for optical microscopy.

Application: Staining method for different kind of cells in blood and bone marrow smears. For the execution of the staining method is required the use of Giemsa solution.

Method:
1) Air dry smears
2) May Grünwald solution, 5 minutes
3) Wash in tap water, 1 minute
4) Giemsa ready to use solution, 15 minutes
5) Wash in tap water, 1-2 minutes
6) Air dry

Results:

Nuclei: violet red purple
Lymphocyte cytoplasm: different blue tonalities
Monocyte cytoplasm: blue-grey
Eosinophil granulocytes (acidophil granules): brick red – orange
Basophil granulocytes (basophil granules): dark violet
Neutrophil granulocytes (neutrophil granules): pink-brown
Erythrocytes: pink –grey