Papanicolaou EA65 Cytoplasmic Staining Solution

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Aim:
Product for the preparation of: gynecological specimens, urine cytology, fine needle specimens, sputum and bronchial washings, to be examined by optical microscopy.

Application:
Cytoplasmic staining solution for Papanicolaou method. For the execution of the staining method is required the use of reagents Papanicolaou Harris hematoxylin and Papanicolaou OG6.

Principle:
A highly selective blue nuclear stain, Harris’ hematoxylin, is combined with EA65 polychromic mixture, a subtle cytoplasmic stain which differentiates cyanophil cells from eosinophil ones. The last ingredient is OG6 solution, which stains keratinized elements.

Method:
1) Ethanol 95°, 2 minutes
2) Distilled water, 2 minutes
3) Harris Hematoxylin, 1 minute
4) Tap water, 5 minutes
5) Ethanol 95°, 15 seconds
6) OG 6, 2 minutes
7) Ethanol 95°, 15 seconds (twice)
8) EA 65, 5 minutes
9) Ethanol 95°, 15 seconds
10) Absolute Ethanol, 30 seconds (twice)
11) Xilene or Bio Clear, 2 minutes (twice)

Results:
Nuclei: Blue-purple
Cyanophil cytoplasm: Blue-green
Eosinophil cytoplasm: Pink
Keratinized cytoplasm: From pink to orange