Papanicolaou OG6 - Cytoplasmic staining

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Product for the preparation of: gynecological specimens, urine cytology, fine needle specimens, sputum and bronchial washings, to be examined by optical microscopy.

Method:

1) Ethanol 95°, 2 minutes
2) Distilled water, 2 minutes
3) Harris Hematoxylin, 1 minute
4) Tap water, 5 minutes
5) Ethanol 95°, 15 seconds
6) OG 6, 2 minutes
7) Ethanol 95°, 15 seconds (twice)
8) EA 65, 5 minutes
9) Ethanol 95°, 15 seconds
10) Absolute Ethanol, 30 seconds (twice)
11) Xilene or Bio Clear, 2 minutes (twice)